Carrascal, LiviaGorton, EllaPardillo-Díaz, RicardoPerez-García, PatriciaGómez-Oliva, RicardoCastro, CarmenNunez-Abades, Pedro2022-09-202022-09-202020-12-19Carrascal L, Gorton E, Pardillo-Díaz R, Perez-García P, Gómez-Oliva R, Castro C, et al. Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons. Antioxidants. 2020 Dec 19;9(12):1307http://hdl.handle.net/10668/4072Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration. To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal development, rats were classified into three groups: Newborn (P2-P7); infantile (P11-P15); and young adult (P20-P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application. In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications in membrane excitability were found. In infantile rats, the input resistance increased and rheobase decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free thiol content. This increase correlated with a time-dependent increase in the glutathione reductase activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore, pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in rat motor neurons increases with age.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Oxidative stressMotor cortexGlutathione levels in the brainPostnatal developmentMembrane excitabilityAmyotrophic lateral sclerosisLipid peroxidationEstrés oxidativoCorteza motoraEsclerosis amiotrófica lateralPeroxidación de lípidoMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::RatsMedical Subject Headings::Organisms::Eukaryota::Animals::Animal Population Groups::Animals, NewbornMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Patch-Clamp TechniquesMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Oxidoreductases Acting on Sulfur Group Donors::Glutathione ReductaseMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Lipid PeroxidationMedical Subject Headings::Anatomy::Nervous System::Central Nervous System::Brain::Prosencephalon::Telencephalon::Cerebrum::Cerebral Cortex::Frontal Lobe::Motor CortexMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Oligopeptides::GlutathioneMedical Subject Headings::Anatomy::Cells::Neurons::Pyramidal CellsMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative StressMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Sulfur Compounds::Sulfhydryl CompoundsMedical Subject Headings::Anatomy::Cells::Neurons::Neurons, Efferent::Motor Neuronsresearch article33352810Acceso abierto10.3390/antiox91213072076-3921PMC7766683