Pérez-Ruiz, MercedesNavarro-Marí, José MaríaBautista-Marín, María-FéPedrosa-Corral, IreneSanbonmatsu-Gámez, SaraCamacho, Ana G.Rojas, JoséRuiz-Ortiz, JorgeRodríguez-Granger, JavierCarrillo, José Antonio2015-11-272015-11-272010-05Pérez-Ruiz M, Navarro-Marí JM, Bautista-Marín MF, Pedrosa-Corral I, Sanbonmatsu-Gámez S, Camacho AG et al. Development and Preliminary Evaluation of a Rapid Oligochromatographic Assay for Specific Detection of New Human Influenza A H1N1 Virus. J Clin Microbiol. 2010 May;48(5):1801-50095-1137http://hdl.handle.net/10668/2086A new oligochromatographic assay, Speed-Oligo Novel Influenza A H1N1, was designed and optimized for the specific detection of the 2009 influenza A H1N1 virus. The assay is based on a PCR method coupled to detection of PCR products by means of a dipstick device. The target sequence is a 103-bp fragment within the hemagglutinin gene. The analytical sensitivity of the new assay was measured with serial dilutions of a plasmid that contained the target sequence, and we determined that down to one copy per reaction of the plasmid was reliably detected. Diagnostic performance was assessed with 103 RNAs from suspected cases (40 positive and 63 negative results) previously analyzed with a reference real-time PCR technique. All positive cases were confirmed, and no false-positive results were detected with the new assay. No cross-reactions were observed when other viral strains or clinical samples with other respiratory viruses were tested. According to these results, this new assay has 100% sensitivity and specificity. The turnaround time for the whole procedure was 140 min. The assay may be especially useful for the specific detection of 2009 H1N1 virus in laboratories not equipped with real-time PCR instrumentsenCromatografíaDiagnóstico por imagenHemaglutininas viralesHumanosVirus de la Influenza AGripe aviarPlásmidosARN viralADN Polimerasa dirigida por ARNSensibilidad y especificidadFactores de tiempoVirologíaReacción en cadena de la polimerasa de transcriptasa inversaMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::ChromatographyMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic ImagingMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Viral Proteins::Viral Structural Proteins::Viral Envelope Proteins::Hemagglutinins, ViralMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Organisms::Viruses::RNA Viruses::Orthomyxoviridae::Influenzavirus A::Influenza A virus::Influenza A Virus, H1N1 SubtypeMedical Subject Headings::Diseases::Virus Diseases::RNA Virus Infections::Orthomyxoviridae Infections::Influenza, HumanMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::PlasmidsMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::RNA::RNA, ViralMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain ReactionMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and SpecificityMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Time::Time FactorsMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Microbiology::Virologyresearch article20220170open access10.1128/JCM.01487-091098-660XPMC2863916