Performance of a Quantitative PCR-Based Assay and Beta-D-Glucan Detection for Diagnosis of Invasive Candidiasis in Very-Low-Birth-Weight Preterm Neonatal Patients (CANDINEO Study)

Tomas Ramos, JoseVillar, SoniaBouza, EmilioBergon-Sendin, ElenaPerez Rivilla, AlfredoTapia Collados, CaridadAndreu, MarianoSantana Reyes, CandelariaIsolina Campos-Herrero, MariaLopez de Heredia, JonLopez Herrera, Maria CruzAnguita Alonso, PalomaRosa Pallas-Alonso, CarmenCuenca-Estrella, ManuelCANDINEO Study Grp2025-01-072025-01-072017-09-010095-1137https://hdl.handle.net/10668/25415An epidemiological, multicenter, noninterventional, observational casecontrol study was conducted to describe the performance of serum beta-D-glucan (BDG) and Candida PCR in blood, serum, and sterile samples for the diagnosis of invasive candidiasis (IC) in very-low-birth-weight (VLBW) preterm neonates and to compare these techniques with culture of samples from blood and other sterile sites. Seventeen centers participated in the study, and the number of episodes analyzed was 159. A total of 9 episodes of IC from 9 patients (7 confirmed and 2 probable) and 150 episodes of suspected sepsis from 117 controls were identified. The prevalence of IC was 5.7% (95% confidence interval [95% CI], 2.1 to 9.3). The mortality was significantly higher in episodes of IC (44.4%) than in the non-IC episodes (11.1%, PenAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/invasive candidiasisserum beta-D-glucanPCRpolymerase chain reactionCare-unit patientsReal-time pcrPediatric-patientsBlood cultureRisk-factorsInfantsInfectionsCandidemiaDnaIdentificationPerformance of a Quantitative PCR-Based Assay and Beta-D-Glucan Detection for Diagnosis of Invasive Candidiasis in Very-Low-Birth-Weight Preterm Neonatal Patients (CANDINEO Study)research article28659321open access10.1128/JCM.00496-171098-660Xhttps://jcm.asm.org/content/jcm/55/9/2752.full.pdf408223700021