Carrasco-Benso, Maria PRivero-Gutierrez, BelenLopez-Minguez, JesusAnzola, AndreaDiez-Noguera, AntoniMadrid, Juan ALujan, Juan AMartinez-Augustin, OlgaScheer, Frank A J LGaraulet, Marta2023-01-252023-01-252016-05-19Carrasco-Benso MP, Rivero-Gutierrez B, Lopez-Minguez J, Anzola A, Diez-Noguera A, Madrid JA, et al. Human adipose tissue expresses intrinsic circadian rhythm in insulin sensitivity. FASEB J. 2016 Sep;30(9):3117-23.http://hdl.handle.net/10668/10148In humans, insulin sensitivity varies according to time of day, with decreased values in the evening and at night. Mechanisms responsible for the diurnal variation in insulin sensitivity are unclear. We investigated whether human adipose tissue (AT) expresses intrinsic circadian rhythms in insulin sensitivity that could contribute to this phenomenon. Subcutaneous and visceral AT biopsies were obtained from extremely obese participants (body mass index, 41.8 ± 6.3 kg/m(2); 46 ± 11 y) during gastric-bypass surgery. To assess the rhythm in insulin signaling, AKT phosphorylation was determined every 4 h over 24 h in vitro in response to different insulin concentrations (0, 1, 10, and 100 nM). Data revealed that subcutaneous AT exhibited robust circadian rhythms in insulin signaling (Pendiabetesglucose toleranceobesityAdipose TissueAdultCircadian RhythmDrug Administration ScheduleHumansInsulinInsulin ResistanceMiddle AgedObesityPhosphorylationProto-Oncogene Proteins c-aktSleepresearch article27256623Restricted AccessAdultoEsquema de medicaciónFosforilaciónInsulinaObesidadPersona de mediana edadProteínas proto-oncogénicas c-aktResistencia a la InsulinaRitmo CircadianoSueñoTejido adiposo10.1096/fj.201600269RR1530-6860PMC5001513https://europepmc.org/articles/pmc5001513https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001513/pdf