Oliva-Olivera, WilfredoCoín-Aragüez, LeticiaSalas, JuliánLhamyani, SaidGentile, Adriana-MarielSarria García, EstebanHmadcha, AbdelkrimZayed, HatemVega-Rioja, AntonioTinahones, Francisco JEl Bekay, Rajaa2016-09-072016-09-072015-12-10Oliva-Olivera W, Coín-Aragüez L, Salas J, Lhamyani S, Gentile AM, Sarria García E, et al. Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PLoS ONE. 2015; 10(12):e0144401http://hdl.handle.net/10668/2403Journal Article; Research Support, Non-U.S. Gov't;OBJECTIVE Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells. MATERIAL AND METHODS We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects. RESULTS TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs. CONCLUSIONS This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.enTejido adiposoAncianoDiferenciación celularAdipogénesisProliferación de la célulaEnsayo de unidades formadoras de coloniasProteínas de unión a ácidos GgrasosCitometría de flujoExpresión génicaInmunofenotipificaciónCélulas del estroma mesenquimatosoMediana edadIsquemia miocárdicaOsteogénesisMedicina regenerativaReacción en cadena de la polimerasa de transcriptasa inversaProteína 1 de unión a los elementos reguladores de esterolesGrasa subcutáneaTimoMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose TissueMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::AgedMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell DifferentiationMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Growth Processes::Cell ProliferationMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Cytological Techniques::Colony-Forming Units AssayMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Carrier Proteins::Fatty Acid-Binding ProteinsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Cytological Techniques::Cell Separation::Flow CytometryMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene ExpressionMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Immunologic Tests::ImmunophenotypingMedical Subject Headings::Anatomy::Cells::Connective Tissue Cells::Stromal Cells::Mesenchymal Stromal CellsMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle AgedMedical Subject Headings::Diseases::Cardiovascular Diseases::Heart Diseases::Myocardial IschemiaMedical Subject Headings::Phenomena and Processes::Musculoskeletal and Neural Physiological Phenomena::Musculoskeletal Physiological Phenomena::Musculoskeletal Physiological Processes::Musculoskeletal Development::Bone Development::OsteogenesisMedical Subject Headings::Disciplines and Occupations::Health Occupations::Medicine::Regenerative MedicineMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain ReactionMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::DNA-Binding Proteins::Basic-Leucine Zipper Transcription Factors::Basic Helix-Loop-Helix Leucine Zipper Transcription Factors::Sterol Regulatory Element Binding Proteins::Sterol Regulatory Element Binding Protein 1Medical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose Tissue::Adipose Tissue, White::Subcutaneous FatMedical Subject Headings::Anatomy::Tissues::Lymphoid Tissue::Thymus GlandMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Differentiation::Adipogenesisresearch article26657132open access10.1371/journal.pone.01444011932-6203PMC4675557