Navarro-Lafuente, FuensantaAdoamnei, EvdochiaArense-Gonzalo, Julian JPrieto-Sanchez, María TSanchez-Ferrer, Maria LParrado, AntonioFernandez, Mariana FSuarez, BeatrizLopez-Acosta, AntoniaSanchez-Guillamon, AntonioGarcia-Marcos, LuisMorales, EvaMendiola, JaimeTorres-Cantero, Alberto MNELA Study group2023-05-032023-05-032022-06-03Navarro-Lafuente F, Adoamnei E, Arense-Gonzalo JJ, Prieto-Sánchez MT, Sánchez-Ferrer ML, Parrado A, et al. Maternal urinary concentrations of bisphenol A during pregnancy are associated with global DNA methylation in cord blood of newborns in the "NELA" birth cohort. Sci Total Environ. 2022 Sep 10;838(Pt 4):156540.http://hdl.handle.net/10668/22512Endocrine disrupting chemicals (EDCs) set a public health risk through disruption of normal physiological processes. The toxicoepigenetic mechanisms of developmental exposure to common EDCs, such as bisphenol A (BPA), are poorly known. The present study aimed to evaluate associations between perinatal maternal urinary concentrations of BPA, bisphenol S (BPS) and bisphenol F (BPF) and LINE-1 (long interspersed nuclear elements) and Alu (short interspersed nuclear elements, SINEs) DNA methylation levels in newborns, as surrogate markers of global DNA methylation. Data come from 318 mother-child pairs of the `Nutrition in Early Life and Asthma´ (NELA) birth cohort. Urinary bisphenol concentration was measured by dispersive liquid-liquid microextraction and ultrahigh performance liquid chromatography with tandem mass spectrometry detection. DNA methylation was quantitatively assessed by bisulphite pyrosequencing on 3 LINEs and 5 SINEs. Unadjusted linear regression analyses showed that higher concentration of maternal urinary BPA in 24th week's pregnancy was associated with an increase in LINE-1 methylation in all newborns (p = 0.01) and, particularly, in male newborns (p = 0.03). These associations remained in full adjusted models [beta = 0.09 (95 % CI = 0.03; 0.14) for all newborns; and beta = 0.10 (95 % CI = 0.03; 0.17) for males], including a non-linear association for female newborns as well (p-trend = 0.003). No associations were found between maternal concentrations of bisphenol and Alu sequences. Our results suggest that exposure to environmental levels of BPA may be associated with a modest increase in LINE-1 methylation -as a relevant marker of epigenomic stability- during human fetal development. However, any effects on global DNA methylation are likely to be small, and of uncertain biological significance.enAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/Bisphenol ADNA methylationEndocrine disruptorsLINE-1Perinatal exposuresAsthmaBenzhydryl CompoundsBirth CohortDNA MethylationEndocrine DisruptorsFemaleFetal BloodHumansInfant, NewbornMaleMaternal ExposurePhenolsPregnancyresearch article35688234open accessAsmaCohorte de nacimientoCompuestos de BencidriloDisruptores endocrinosEmbarazoExposición maternaFemeninoFenolesMasculinoMetilación de ADNSangre fetalRecién nacido10.1016/j.scitotenv.2022.1565401879-1026https://doi.org/10.1016/j.scitotenv.2022.156540