Altea-Manzano, PatriciaUnciti-Broceta, Juan DiegoCano-Cortes, VictoriaRuiz-Blas, María PazValero-Griñan, TeresaDiaz-Mochon, Juan JoseSanchez-Martin, Rosario2023-01-252023-01-252017-05-17http://hdl.handle.net/10668/11202To develop an efficient nanotechnology fluorescence-based method to track cell proliferation to avoid the limitations of current cell-labeling dyes. Synthesis, PEGylation, bifunctionalization and labeling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterized and assessed for in vitro long-term monitoring of cell proliferation. The optimization and validation of this method to track long-term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labeled NPs has been successfully developed. [Formula: see text].enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/cell proliferationcell trackingflow cytometryfluorescent nanoparticlesnanofectionCell CycleCell Line, TumorCell ProliferationCell TrackingFeasibility StudiesFlow CytometryFluorescenceFluorescent DyesHumansLeukocytes, MononuclearNanoparticlesPolystyrenesReproducibility of ResultsStaining and LabelingSurface PropertiesTransfectionresearch article28513331open access10.2217/nnm-2017-01181748-6963https://bradscholars.brad.ac.uk/bitstream/10454/13342/1/ManuscriptProliferation_Nanomedicine_Final%20Revision_resubmission2%20%281%29.pdf