Palacín, ArantxaRivas, Luis A.Gómez-Casado, CristinaAguirre, JacoboTordesillas, LeticiaBartra, JoanBlanco, CarlosCarrillo, TeresaCuesta-Herranz, JavierCumplido Bonny, José A.Flores, EnriqueGarcía-Alvarez-Eire, Mar G.García-Nuñez, IgnacioFernández, Francisco J.Gamboa, PedroMuñoz, RosaSánchez-Monge, RosaTorres, MariaVarela Losada, SusanaVillalba, MayteVega, FranciscoParro, VictorBlanca, MiguelSalcedo, GabrielDíaz-Perales, Araceli2013-12-042013-12-042012-09-07Palacín A, Rivas LA, Gómez-Casado C, Aguirre J, Tordesillas L, Bartra J, et al. The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray. PLoS ONE. 2012; 7(9):e44088http://hdl.handle.net/10668/1409Multicenter Study; Research Support, Non-U.S. Gov't;Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.enEspañaGeografíaReacciones cruzadasHipersensibilidad a los alimentosInmunizaciónInmunoensayoInmunoglobulina EPeso molecularProteínas de plantasPolenAnálisis por matrices de proteínasMedical Subject Headings::Named Groups::Persons::Age Groups::AdolescentMedical Subject Headings::Named Groups::Persons::Age Groups::AdultMedical Subject Headings::Named Groups::Persons::Age Groups::ChildMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Check Tags::MaleMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Young AdultMedical Subject Headings::Geographicals::Geographic Locations::Europe::SpainMedical Subject Headings::Phenomena and Processes::Immune System Phenomena::Immune System Processes::Antigen-Antibody Reactions::Cross ReactionsMedical Subject Headings::Diseases::Immune System Diseases::Hypersensitivity::Hypersensitivity, Immediate::Food HypersensitivityMedical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::FoodMedical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::FruitMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Earth Sciences::GeographyMedical Subject Headings::Named Groups::Persons::Age Groups::Adult::Middle AgedMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::ImmunizationMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::ImmunoassayMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin EMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::Molecular WeightMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant ProteinsMedical Subject Headings::Anatomy::Plant Structures::Plant Components, Aerial::Flowering Tops::Flowers::Germ Cells, Plant::PollenMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Microchip Analytical Procedures::Microarray Analysis::Protein Array Analysisresearch article22970164open access10.1371/journal.pone.00440881932-6203PMC3436791