Milian-Linares, M. CarmenLemus-Conejo, AnaMar-Yust, M.Pedroche, JustoCarrillo-Vico, AntonioMilian, FranciscoMontserrat-de-la-Paz, Sergio2023-02-122023-02-122018-06-08Millan-Linares M.C., Lemus-Conejo A, Mar Yust M, Pedroche J, Carrillo-Vico A, Millan F, et al. GPETAFLR, a novel bioactive peptide from Lupinus angustifolius L. protein hydrolysate, reduces osteoclastogenesis. J. funct. food. 2018-Jun-08 (47) 299-3031756-4646http://hdl.handle.net/10668/18818The effect of GPETAFLR, a peptide isolated from Lupinus angustifolius L. protein hydrolysate (LPH), on osteoclastogenesis was investigated. Human osteoclasts generated from monocytes were used to analyse the effects of GPETAFLR (50-100 mu g/mL) on osteoclastogenesis using TRAP reaction, RT-qPCR, and ELISA procedures. LPS enhanced TRAP activity and the expression of osteoclast marker genes (TRAP, OSCAR, RANK, and CATHK) while downregulated the expression of OPG gene in human monocyte-derived osteoclasts. These effects were reduced with GPETAFLR. Moreover, LPS increased the release of osteoclastogenic cytokines (TNF-alpha, IL-1 beta and IL-6) meanwhile GPETAFLR increased the release of anti-osteoclastogenic cytokines (IL-4 and IL-10) in the medium of human monocyte-derived osteoclasts. For the first time, we show that plant peptides from lupine protein hydrolysates have anti-osteoclastogenic activity. These exciting findings open opportunities for developing nutritional strategies with Lupinus angustifolius L. as dietary source of plant proteins, notably GPETAFLR, to prevent development and progression of osteoclast-related diseases.enPeptideProtein hydrolysateLupineOsteoclastOsteoporosisBoneOsteoporosisInhibitionRankl/opgPathwayInterleukin-10OsteoclastsProtein HydrolysatesLupinusMonocytesLipopolysaccharidesresearch articleRestricted AccessOsteoclastosLupinusMonocitosOsteogénesisEnfermedadFactor de necrosis tumoral alfaEnsayo de inmunoadsorción enzimática10.1016/j.jff.2018.05.069https://digital.csic.es/bitstream/10261/178457/4/PostP_2018_JFF_V47_P299.pdf439019200033