Naren, GaowaLarsson, WeraBenitez-Martin, CarlosLi, ShimingPérez-Inestrosa, EzequielAlbinsson, BoAndréasson, Joakim2022-09-272022-09-272021-04-15Naren G, Larsson W, Benitez-Martin C, Li S, Pérez-Inestrosa E, Albinsson B, et al. Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging. Chem Sci. 2021 Apr 15;12(20):7073-7078http://hdl.handle.net/10668/4165A water soluble diarylethene (DAE) derivative that displays exceptionally intense fluorescence from the colorless open form has been synthesized and characterized using UV/vis spectroscopy and fluorescence microscopy. We show that the bright emission from the open form can be rapidly switched using amplitude modulated red light, that is, by light at wavelengths longer than those absorbed by the fluorescent species. This is highly appealing in any context where undesired background fluorescence disturbs the measurement, e.g., the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.enAtribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/FluorescenceMicroscopy, fluorescenceFluorescent dyesUVFluorescenciaMicroscopía fluorescenteColorantes fluorescentesRayos ultravioletaMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microscopy::Microscopy, FluorescenceMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Photometry::Luminescent Measurements::Fluorometry::Spectrometry, FluorescenceMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Specialty Uses of Chemicals::Coloring Agents::Fluorescent DyesMedical Subject Headings::Phenomena and Processes::Chemical Phenomena::Physicochemical Phenomena::SolubilityMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Optical Phenomena::LightMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Radiation::Electromagnetic Radiation::Light::Ultraviolet RaysMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::MicroscopyMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Culture Techniquesresearch article34123335Acceso abierto10.1039/d1sc01071a2041-6539PMC8153230