Garcia-Fernandez, EmilioGonzalez-Garcia, M CarmenPernagallo, SalvatoreRuedas-Rama, Maria JFara, Mario ALópez-Delgado, Francisco JDear, James WIlyine, HughRess, CristinaDíaz-Mochón, Juan JOrte, Angel2023-02-082023-02-082019-11-27http://hdl.handle.net/10668/14751A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Fluorescent DyesHumansLimit of DetectionMicroRNAsOptical Imagingresearch article31774422open access10.1039/c9cc08069d1364-548Xhttps://pubs.rsc.org/en/content/articlepdf/2019/cc/c9cc08069d