Linares-Gonzalez, LauraRodenas-Herranz, TeresaCampos, FernandoRuiz-Villaverde, RicardoCarriel, Víctor2025-01-072025-01-072021-09-302075-1729https://hdl.handle.net/10668/25991Reconstruction of skin defects is often a challenging effort due to the currently limited reconstructive options. In this sense, tissue engineering has emerged as a possible alternative to replace or repair diseased or damaged tissues from the patient's own cells. A substantial number of tissue-engineered skin substitutes (TESSs) have been conceived and evaluated in vitro and in vivo showing promising results in the preclinical stage. However, only a few constructs have been used in the clinic. The lack of standardization in evaluation methods employed may in part be responsible for this discrepancy. This review covers the most well-known and up-to-date methods for evaluating the optimization of new TESSs and orientative guidelines for the evaluation of TESSs are proposed.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/biochemical methodsbiomechanical evaluationhistological techniquesquality controlskin tissue engineeringresearch article34685402open access10.3390/life11101033PMC8541591https://www.mdpi.com/2075-1729/11/10/1033/pdf?version=1633344189https://pmc.ncbi.nlm.nih.gov/articles/PMC8541591/pdf