Martinez-Tellez, BorjaSanchez-Delgado, GuillermoGarcia-Rivero, YolandaAlcantara, Juan M AMartinez-Avila, Wendy DMuñoz-Hernandez, Maria VOlza, JosuneBoon, Mariëtte RRensen, Patrick C NLlamas-Elvira, Jose MRuiz, Jonatan R2023-01-252023-01-252017-11-021664-042Xhttp://hdl.handle.net/10668/11828Brown adipose tissue (BAT) activity is induced when humans are exposed to cold. Therefore, cold exposure prior to the 18F-FDG-PET/CT scan is used as a tool to quantify BAT. Several cooling protocols, including fixed and personalized ones are currently in use. The aim of the present study was to determine the effect of a new personalized cooling protocol where the shivering threshold was measured on a separate day, on BAT volume and activity in young adults. A total of 47 adults (n = 28 women) aged 22 ± 2 years participated in the study. We determined participants' shivering threshold (visually and self-reported) using a water perfused cooling vest in an air-conditioned cold room. 48-72 h later, participants wore the cooling vest set at ~4°C above the shivering threshold for 60 min prior to injection of 18F-FDG and ~5°C above the shivering threshold for ~60 min after injection, until PET/CT scan. We quantified BAT following BARCIST 1.0 recommendations. We identified 40 participants (85%, n = 25 women) as PET+ and 7 (n = 3 women) as PET-. The PET+ group presented significantly higher BAT volume and activity than PET- group (all PenAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/PET/CT scanbody temperaturecooling vestglucose uptakethermal perceptionresearch article29163207open access10.3389/fphys.2017.00863PMC5673647https://www.frontiersin.org/articles/10.3389/fphys.2017.00863/pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673647/pdf