Velázquez-Suárez, CristinaCebrián, RubénGasca-Capote, CarmenSorlózano-Puerto, AntonioGutiérrez-Fernández, JoséMartínez-Bueno, ManuelMaqueda, MercedesValdivia, Eva2023-01-102023-01-102021-07-30Velázquez-Suárez C, Cebrián R, Gasca-Capote C, Sorlózano-Puerto A, Gutiérrez-Fernández J, Martínez-Bueno M, et al. Antimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus. Antibiotics. 2021 Jul 30;10(8):925http://hdl.handle.net/10668/4557The treatment and hospital-spread-control of methicillin-resistant Staphylococcus aureus (MRSA) is an important challenge since these bacteria are involved in a considerable number of nosocomial infections that are difficult to treat and produce prolonged hospitalization, thus also increasing the risk of death. In fact, MRSA strains are frequently resistant to all β-lactam antibiotics, and co-resistances with other drugs such as macrolides, aminoglycosides, and lincosamides are usually reported, limiting the therapeutical options. To this must be added that the ability of these bacteria to form biofilms on hospital surfaces and devices confer high antibiotic resistance and favors horizontal gene transfer of genetic-resistant mobile elements, the spreading of infections, and relapses. Here, we genotypically and phenotypically characterized 100 clinically isolated S. aureus for their resistance to 18 antibiotics (33% of them were OXA resistant MRSA) and ability to form biofilms. From them, we selected 48 strains on the basis on genotype group, antimicrobial-resistance profile, and existing OXA resistance to be assayed against bacteriocin AS-48. The results showed that AS-48 was active against all strains, regardless of their clinical source, genotype, antimicrobial resistance profile, or biofilm formation capacity, and this activity was enhanced in the presence of the antimicrobial peptide lysozyme. Finally, we explored the effect of AS-48 on formed S. aureus biofilms, observing a reduction in S. aureus S-33 viability. Changes in the matrix structure of the biofilms as well as in the cell division process were observed with scanning electron microscopy in both S-33 and S-48 S. aureus strains.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Staphylococcus aureus MRSAEnterocin AS-48Antibiotic resistanceBiofilmsAminoglycosidesMacrolidesMethicillinInfectionsBacteriocinsElectron microscopyGenotypeStaphylococcus aureus resistente a meticilinaFarmacorresistencia microbianaBiopelículasAminoglicósidosMacrólidosMeticilinaInfeccionesBacteriocinasMicroscopía electrónicaGenotipoMedical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureus::Methicillin-Resistant Staphylococcus aureusMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial AgentsMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Glycosides::AminoglycosidesMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Lactones::MacrolidesMedical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 1-Ring::Pyrrolidines::LincosamidesMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::Amides::Lactams::beta-Lactams::Penicillins::MethicillinMedical Subject Headings::Diseases::Bacterial Infections and Mycoses::Infection::Cross InfectionMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Bacterial Proteins::BacteriocinsMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Glycoside Hydrolases::MuramidaseMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Recombination, Genetic::Gene Transfer, HorizontalMedical Subject Headings::Phenomena and Processes::Physical Phenomena::Elementary Particles::ElectronsMedical Subject Headings::Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, BacterialMedical Subject Headings::Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Staphylococcaceae::Staphylococcus::Staphylococcus aureusMedical Subject Headings::Phenomena and Processes::Microbiological Phenomena::BiofilmsMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::GenotypeMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Cell DivisionMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::Disease Attributes::RecurrenceMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Patient Care::Hospitalizationresearch article34438974open access10.3390/antibiotics100809252079-6382PMC8388780