Breitling, RainerAvbelj, MartinaBilyk, OksanaCarratore, Francesco DelFilisetti, AlessandroHanko, Erik K RIorio, MariannaRedondo, Rosario PérezReyes, FernandoRudden, MichelleSeveri, EmmanueleSlemc, LucijaSchmidt, KamilaWhittall, Dominic RDonadio, StefanoGarcía, Antonio RodríguezGenilloud, OlgaKosec, GregorDe Lucrezia, DavidePetković, HrvojeThomas, GavinTakano, Eriko2023-02-092023-02-092021http://hdl.handle.net/10668/17855Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/actinobacteriaactinomycetebiosynthesisbiotechnologysynthetic biologyActinobacteriaIndustrial MicrobiologyMetabolic EngineeringSynthetic Biologyresearch article34057181open access10.1093/femsle/fnab0601574-6968PMC8195692https://academic.oup.com/femsle/article-pdf/368/10/fnab060/38609453/fnab060.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195692/pdf