de la Rosa, ManuelPerez, MercedesCarazo, ConcepcionPareja, LuciaPeis, Jose IgnacioHernandez, Fernando2016-01-082016-01-081992-04de la Rosa M, Perez M, Carazo C, Pareja L, Peis JI, Hernandez F. New Granada Medium for detection and identification of group B streptococci. J Clin Microbiol. 1992; 30(4):1019-210095-1137http://hdl.handle.net/10668/2121Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.enMetotrexatoMedios de cultivoPigmentaciónTécnicas bacteriológicasGranadaAndalucíaMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture MediaMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Evaluation Studies as TopicMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Pteridines::Pterins::Aminopterin::MethotrexateMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::PigmentationMedical Subject Headings::Organisms::Bacteria::Gram-Positive Bacteria::Gram-Positive Cocci::Streptococcaceae::Streptococcus::Streptococcus agalactiaeMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniquesresearch article1572958open access1098-660XPMC265207