Lisbona-González, Maria JesúsMuñoz-Soto, EstherReyes-Botella, CandelaOlmedo-Gaya, Maria VictoriaDiaz-Castro, JavierMoreno-Fernandez, Jorge2022-10-032022-10-032021-08-13Lisbona-González MJ, Muñoz-Soto E, Reyes-Botella C, Olmedo-Gaya MV, Diaz-Castro J, Moreno-Fernandez J. Study of the Antimicrobial Effect of an Ethanolic Extract of Propolis in Periodontal Disease. Appl. Sci. 2021;11(16):7463http://hdl.handle.net/10668/4209Periodontal disease encompasses gingivitis and periodontitis and is one of the most common chronic infections in the adult population. This study aimed to evaluate the influence of Spanish propolis extract (EEP) on the effect of the clinical and microbiological parameters as an adjuvant to scaling and root planning in patients undergoing supportive periodontal therapy (SPT). Forty chronic periodontitis patients were randomly assigned into two groups for the treatment. In the control group (n = 20), the sites were treated by scaling and root planing followed by gingival irrigation with physiological saline and in the test group (n = 20), the sites were treated by scaling and root planing followed by subgingival placement of EEP. At baseline (BL), bleeding on probing positive (BOP+) sites with probing pocket (PPD) ≥ 4 mm were defined as study sites. Plaque index, PPD, BOP, clinical attachment level (CAL), and subgingival plaque were evaluated at BL and 1 month later. The results showed a significant clinical improvement (p < 0.05) in the PPD, CAL and BOP+ comparing them with BL and one month after the periodontal treatment and a significant reduction (p < 0.05) for Tannerella forsythensis, Porphyromonas gingivalis, Prevotella intermedia and Treponema denticola in both groups. In addition, the improvement of clinical parameters was observed with subgingival use of EEP and also statistically significant differences between groups were observed (p < 0.05) such as reductions of BOP+ % and reduced counts of T. forsythensis and P. gingivalis, considered as the “key pathogens” for the periodontal diseases. Our results suggest prophylactic and therapeutic potential for EEP against periodontal diseases, improving clinical parameters, reducing gingival bleeding and decreasing bacterial counts of T. forsythensis and P. gingivalis. The subgingival use of EEP represents a promising modality as an adjuvant in periodontal therapy to avoid microbial resistance and other adverse effects.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/PropolisOral healingPeriodontal diseaseAntimicrobial effectEnfermedades periodontalesAntiinfecciososInfección persistentePeriodontitis crónicaGrupos controlPrópolisMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Persons::Persons::Age Groups::AdultMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Dentistry::Periodontics::Dental Prophylaxis::Dental Scaling::Root PlaningMedical Subject Headings::Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Anaerobic Bacteria::Gram-Negative Anaerobic Straight, Curved, and Helical Rods::Spirochaetaceae::Treponema::Treponema denticolaMedical Subject Headings::Organisms::Bacteria::Bacteroidetes::Bacteroidaceae::Porphyromonas::Porphyromonas gingivalisMedical Subject Headings::Organisms::Bacteria::Bacteroidetes::Bacteroidaceae::Prevotella::Prevotella intermediaMedical Subject Headings::Diseases::Stomatognathic Diseases::Mouth Diseases::Periodontal Diseases::Periodontitis::Chronic PeriodontitisMedical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Resins, Plant::PropolisMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Antigens, Bacterial::TuberculinMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Bacteriological Techniques::Bacterial LoadMedical Subject Headings::Diseases::Stomatognathic Diseases::Mouth Diseases::Periodontal Diseases::Gingival Diseases::Gingivitisresearch articleAcceso abiertohttps://doi.org/10.3390/app111674632076-3417