Mesas, CristinaMartínez, RosarioOrtíz, RaúlGalisteo, MilagrosLópez-Jurado, MaríaCabeza, LauraPerazzoli, GloriaMelguizo, ConsolaciónPorres, Jesús M.Prados, Jose2022-04-052022-04-052021-02-09Mesas C, Martínez R, Ortíz R, Galisteo M, López-Jurado M, Cabeza L, et al.. Antitumor Effect of the Ethanolic Extract from Seeds of Euphorbia lathyris in Colorectal Cancer. Nutrients. 2021 13(2):566.http://hdl.handle.net/10668/3518Results have been protected by the patent application Ministerio de Ciencia e Innovación P202030454.The seeds of Euphorbia lathyris have been used in traditional medicine to treat various medical conditions. However, neither all of their active biocompounds nor the molecular mechanisms underlying their therapeutic effects have been described. A new ethanolic extract of defatted flour from mature seeds of Euphorbia lathyris showed a high total polyphenol content and significant antioxidant activity. Chromatographic analysis showed that esculetin, euphorbetin, gaultherin, and kaempferol-3-rutinoside were the most abundant polyphenolic bioactive compounds. Antiproliferative assays showed a high and selective antitumor activity against colon cancer cell lines (T84 and HCT-15). In addition, a significant antiproliferative activity against glioblastoma multiforme cells was also demonstrated. Its mechanism of action to induce cell death was mediated by the overexpression of caspases 9, 3, and 8, and by activation of autophagy. Interestingly, a reduction in the migration capacity of colon cancer cells and a significant antiangiogenic effect on human umbilical vein endothelial cells were also demonstrated. Finally, the extract significantly reduced the subpopulations of cancer stem cells. This extract could be the basis to develop new therapeutic strategies for the treatment of colon cancer, although further experiments will be necessary to determine its in vivo effects.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Colon cancerEuphorbia lathyrisEthanolic extractApoptosisAntiangiogenic effectCancer stem cellsNeoplasias del colonProliferación celularEtanolPolifenolesMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Antineoplastic Agents::Antineoplastic Agents, PhytogenicMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Molecular Mechanisms of Pharmacological Action::AntioxidantsMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, TumorMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Gastrointestinal Neoplasms::Intestinal Neoplasms::Colorectal Neoplasms::Colonic NeoplasmsMedical Subject Headings::Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Euphorbiaceae::EuphorbiaMedical Subject Headings::Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Germ Cell and Embryonal::Neuroectodermal Tumors::Neoplasms, Neuroepithelial::Glioma::Astrocytoma::GlioblastomaMedical Subject Headings::Anatomy::Cells::Epithelial Cells::Endothelial Cells::Human Umbilical Vein Endothelial CellsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Phenomena and Processes::Circulatory and Respiratory Physiological Phenomena::Cardiovascular Physiological Phenomena::Cardiovascular Physiological Processes::Neovascularization, PhysiologicMedical Subject Headings::Chemicals and Drugs::Pharmaceutical Preparations::Plant ExtractsMedical Subject Headings::Anatomy::Plant Structures::Plant Components, Aerial::Fruit::SeedsAntitumor Effect of the Ethanolic Extract from Seeds of Euphorbia lathyris in Colorectal Cancerresearch article33572111Acceso abierto10.3390/nu130205662072-6643PMC7915714