Sánchez-Hernández, SilviaThéron, LaëtitiaJiménez-Barrios, PabloOlalla-Herrera, ManuelRecio, IsidraMiralles, Beatriz2022-06-302022-06-302021-04-18Sánchez-Hernández S, Théron L, Jiménez-Barrios P, Olalla-Herrera M, Recio I, Miralles B. Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers. Foods. 2021 Apr 18;10(4):887http://hdl.handle.net/10668/3724Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers' body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother's condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data.enAtribución 4.0 InternacionalAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Human milkMALDI mass spectrometryBody mass indexIn vitro infant digestionBioactive proteinsLeche humanaEspectrometría de masa por láser de matriz asistida de ionización desorciónÍndice de masa corporalRecién nacidoMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Persons::Persons::Age Groups::Infant::Infant, NewbornMedical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Milk, HumanMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Dietary Proteins::Milk ProteinsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::PeptidesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass SpectrometryMedical Subject Headings::Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::LactationMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Nutrition Processes::DigestionMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Signs and Symptoms::Body Weight::OverweightMedical Subject Headings::Anatomy::Fluids and Secretions::Bodily Secretions::Colostrumresearch article33919642Acceso abierto10.3390/foods100408872304-8158PMC8074095