Ladehesa, L.Castro, M. G.Lopez-Pedrera, C.Ortega, R.Pedraza, S.del Rio, M.Abalos-Aguilera, M. C.Ruiz-Limon, P.Perez-Sanchez, C.Ibanez-Costa, A.Parra-Manso, Y.Barbarroja, N.Arias-de la Rosa, I.Font, P.Escudero, A.Castano, J. P.Luque, R. M.Collantes, E.Jimenez-Gomez, Y.2025-01-072025-01-072022-01-09Ladehesa L, Castro M, Lopez-Pedrera C, et al FRI0157 Altered expression of the spliceosome components in leukocytes subsets from patients with ankylosing spondylitis: association to disease pathogenesis and therapeutic responseAnnals of the Rheumatic Diseases 2018;77:621-6220003-4967https://hdl.handle.net/10668/28325Ankylosing spondylitis (AS) is a chronic inflammatory disease whose etiopathogenesis remains incompletely understood. Splicing, a posttranscriptional process essential for RNA maturation, has recently been implicated in several human diseases through pathological deregulation of the spliceosome; however, alterations of the spliceosome and their modulation by therapeutic responses have not yet been described in AS. The objectives of this study were: (1) to evaluate the potential deregulation of the spliceosome in leukocytes from AS patients and its involvement in disease pathophysiology, and (2) to analyze the in vivo effects of anti-TNF drugs on spliceosome components in these cells. A cross-sectional study was conducted with 32 AS patients and 29 healthy donors (HDs), and a three-month longitudinal study was performed in eight AS patients undergoing anti-TNFα therapy. Disease activity was assessed using the BASDAI index, along with CRP and ESR levels; physical function was measured by BASFI, spinal mobility by BASMI, and structural damage by mSASSS. The expression of selected components of the major (n=12) and minor (n=4) spliceosomes, as well as splicing factors (n=28), was evaluated in purified leukocytes using Fluidigm technology, while inflammatory marker expression was assessed in parallel by RT-PCR.enSpondylitis, AnkylosingSpliceosomesCross-Sectional StudiesTumor Necrosis Factor InhibitorsLongitudinal StudiesRNA Splicing FactorsReverse Transcriptase Polymerase Chain ReactionLeukocytesconference outputRestricted AccessEmpalmosomasDonantes de tejidosEspondilitis AnquilosanteFactores de empalme de ARN10.1136/annrheumdis-2018-eular.45691468-2060https://ard.bmj.com/content/annrheumdis/77/Suppl_2/621.3.full.pdf444351001618