Villatoro, Antonio JClaros, SilviaFernandez, VivianaAlcoholado, CristinaFariñas, FernandoMoreno, AntonioBecerra, JoseAndrades, Jose A2023-01-252023-01-252018-03-27Villatoro AJ, Claros S, Fernández V, Alcoholado C, Fariñas F, Moreno A, et al. Safety and efficacy of the mesenchymal stem cell in feline eosinophilic keratitis treatment. BMC Vet Res. 2018 Mar 27;14(1):116.http://hdl.handle.net/10668/12281Feline eosinophilic keratitis (FEK) is a chronic keratopathy caused by a suspected immune mediated response to an unknown antigenic stimulus. The purpose of this study was to investigate the safety and therapeutic effects of allogeneic feline adipose-derived mesenchymal stromal cells (fAd-MSCs) implanted subconjunctival around the ocular surface lesion in five cats with FEK refractory to current available treatments. FEK was diagnosed by clinical appearance and evidence of eosinophil and/or mast cells in corneal cytology. Each animal was treated with two applications of 2 × 106 million of fAd-MSCs 2 months apart. Ocular surface integrity was assessed before treatment and at 1, 3, 6 and 11 months after treatment. Clinical signs showed a significant change during the follow-up with resolution of the corneal and conjunctiva lesions and there were no signs of regression or worsening. Implanted cells were well-tolerated and effective reducing clinical signs of FEK with a sustained effect during the study period. None of the animals showed systemic or local complications during the study. To our knowledge, this is the first time in literature that local implantation of allogeneic fAd-MSCs has been found as an effective therapeutic alternative to treat cats with FEK.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Adipose mesenchymal stem cellAllogeneic cell therapyCatFeline eosinophilic keratitisFeline herpes virusLacrimal glandCentro Andaluz de Nanomedicina y Biotecnología (BIONAND)AnimalsCat DiseasesCatsEosinophiliaFemaleKeratitisMaleMesenchymal Stem Cell TransplantationTreatment Outcomeresearch article29587744open accessQueratitis eosinofílica felinaOjoCélulas madre mesenquimalesTejido adiposoInmunomodulaciónImplante subconjuntivalIntegridad de la superficie ocularSignos clínicos10.1186/s12917-018-1413-41746-6148PMC5870249https://doi.org/10.1186/s12917-018-1413-4https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5870249/pdf