Pacios, OlgaFernandez-Garcia, LauraBleriot, InesBlasco, LuciaAmbroa, AntonLopez, MariaOrtiz-Cartagena, ConchaCuenca, Felipe FernandezOteo-Iglesias, JesusPascual, AlvaroMartinez-Martinez, LuisDomingo-Calap, PilarTomas, Maria2023-05-032023-05-032021-12-17Pacios O, Fernández-García L, Bleriot I, Blasco L, Ambroa A, López M, et al. Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13. Viruses. 2021 Dec 21;14(1):6http://hdl.handle.net/10668/21597Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Klebsiella pneumoniaeL-shaped tail fiberGenomic annotationHoming endonucleasesLytic phagesBacteriolysisGenes, viralGenome, viralHost specificityHumansKlebsiella infectionsKlebsiella pneumoniaeMyoviridaePhage therapyPhenotypePhylogenyViral proteinsWhole genome sequencingresearch article35062209open accessBacteriólisisEspecificidad del huéspedFenotipoFilogeniaGenes viralesGenoma viralInfecciones por Klebsiella10.3390/v140100061999-4915PMC8778798https://www.mdpi.com/1999-4915/14/1/6/pdf?version=1640088707https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778798/pdf