Vítor, Alexandra C.Huertas, PabloLegube, Gaëllede Almeida, Sérgio F.2022-07-042022-07-042020-02-21Vítor AC, Huertas P, Legube G, de Almeida SF. Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox. Front Mol Biosci. 2020 Feb 21;7:24http://hdl.handle.net/10668/3734To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/DNA repairHomologous recombination (HR)Non-homologous DNA end joiningChromatinDNA damageReparación del ADNRecombinación homólogaReparación del ADN por unión de extremidadesCromatinaDaño del ADNRoturas del ADN de doble cadenaMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Damage::DNA Breaks::DNA Breaks, Double-StrandedMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA RepairMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::Recombination, Genetic::Homologous RecombinationMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Repair::DNA End-Joining RepairMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Structures::Chromosome Structures::ChromatinMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA DamageMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Cycle CheckpointsMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNAMedical Subject Headings::Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Genetics::Genomicsreview article32154266Acceso abierto10.3389/fmolb.2020.000242296-889XPMC7047327