Rivero-Juarez, AntonioRisalde, María AGortázar, ChristianLopez-Lopez, PedroBarasona, Jose AFrias, MarioCaballero-Gomez, Javierde la Fuente, JoséRivero, Antonio2025-01-072025-01-072021-07-091664-302Xhttps://hdl.handle.net/10668/25480Little is known about the role of ticks in maintaining highly prevalent zoonotic viruses in wildlife, such as hepatitis E virus (HEV), which do not require ticks for transmission between animals and humans. In this cross-sectional study, adult female ticks were collected from Eurasian wild boar (Sus scrofa) in autumn 2015 in Spain. HEV RNA in both ticks and wild boar was evaluated by RT-qPCR. Twenty-nine adult Hyalomma lusitanicum ticks were collected from 29 wild boars. HEV RNA was detected in a total of 10 tick (34.4%) and 11 wild boar serum samples (37.9%). In two cases, detectable HEV RNA was found in a wild boar but not in the tick collected from them. In contrast, one HEV-positive tick was collected from an HEV-negative wild boar. All viral sequences were consistent with genotype 3f. We describe for the first time the presence of HEV RNA in adult Hyalomma lusitanicum ticks.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Hyalomma lusitanicumepidemiologyhepatitis Etickwild boarresearch article34305854open access10.3389/fmicb.2021.692147PMC8299481https://www.frontiersin.org/articles/10.3389/fmicb.2021.692147/pdfhttps://pmc.ncbi.nlm.nih.gov/articles/PMC8299481/pdf