Ramírez-Tortosa, César LAlonso-Calderón, RubénGálvez-Navas, José MaríaPérez-Ramírez, CristinaQuiles, José LuisSánchez-Rovira, PedroJiménez-Morales, AlbertoRamírez-Tortosa, MCarmen2023-05-032023-05-032022-11-022072-6694http://hdl.handle.net/10668/20924To demonstrate the value of hypoxia-inducible factor-1α (HIF-1α) in predicting response in patients with breast cancer receiving standard neoadjuvant chemotherapy (NAC). Ninety-five women enrolled in two prospective studies underwent biopsies for the histopathological diagnosis of breast carcinoma before receiving NAC, based on anthracyclines and taxanes. For expression of HIF-1α, EGFR, pAKT and pMAPK, tumor samples were analyzed by immunohistochemistry in tissues microarrays. Standard statistical methods (Pearson chi-square test, Fisher exact test, Kruskal-Wallis test, Mann-Whitney test and Kaplan-Meier method) were used to study the association of HIF-1α with tumor response, survival and other clinicopathologic variables/biomarkers. HIF-1α expression was positive in 35 (39.7%) cases and was significantly associated to complete pathological response (pCR) (p = 0.014). HIF-1α expression was correlated positively with tumor grade (p = 0.015) and Ki-67 expression (p = 0.001) and negativity with progesterone receptors (PR) (p = 0.04) and luminal A phenotype expression (p = 0.005). No correlation was found between HIF-1α expression and EGFR, pAKT and pMAPK. In terms of survival, HIF-1α expression was associated with a significantly shorter disease-free survival (p = 0.013), being identified as an independent prognostic factor in multivariate analysis. Overexpression of HIF-1α is a predictor of pCR and shorter DFS; it would be valuable to confirm these results in prospective studies.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/breast cancerhypoxia-inducible factor 1neoadjuvant chemotherapypathological complete responseprognostic factorresearch article36358811open access10.3390/cancers14215393PMC9656699https://www.mdpi.com/2072-6694/14/21/5393/pdf?version=1667376707https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656699/pdf