Campos-Cuerva, RafaelFernandez-Muñoz, BeatrizFarfan Lopez, FranciscoPereira Arenas, SheilaSantos-Gonzalez, MonicaLopez-Navas, LuisAlaminos, MiguelCampos, AntonioMuntane, JordiCepeda-Franco, CarmenGomez-Bravo, Miguel Angel2023-01-252023-01-252019-02-21Campos-Cuerva R, Fernández-Muñoz B, Farfán López F, Pereira Arenas S, Santos-González M, Lopez-Navas L, et al. Nanostructured fibrin agarose hydrogel as a novel haemostatic agent. J Tissue Eng Regen Med. 2019 Apr;13(4):664-673.http://hdl.handle.net/10668/13608Blood loss remains a major concern during surgery and can increase the morbidity of the intervention. The use of topical haemostatic agents to overcome this issue therefore becomes necessary. Fibrin sealants are promising haemostatic agents due to their capacity to promote coagulation, but their effectiveness and applicability need to be improved. We have compared the haemostatic efficacy of a novel nanostructured fibrin-agarose hydrogel patch, with (c-NFAH) or without cells (a-NFAH), against two commercially available haemostatic agents in a rat model of hepatic resection. Hepatic resections were performed by making short or long incisions (mild or severe model, respectively), and haemostatic agents were applied to evaluate time to haemostasis, presence of haematoma, post-operative adhesions to adjacent tissues, and inflammation factors. We found a significantly higher haemostatic success rate (time to haemostasis) with a-NFAH than with other commercial haemostatic agents. Furthermore, other relevant outcomes investigated were also improved in the a-NFAH group, including no presence of haematoma, lower adhesions, and lower grades of haemorrhage, inflammation, and necrosis in histological analysis. Overall, these findings identify a-NFAH as a promising haemostatic agent in liver resection and likely in a range of surgical procedures.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/bleedingfibrin agarose hydrogelfibrin sealanthaemostasishaemostatic agentliver resectionnanostructured biomaterialsurgeryAnimalsFibrinHemorrhageHemostaticsHydrogelsInflammationLiverMaleNanostructuresNecrosisRats, WistarSepharoseresearch article30793853open accessAnimalesHemorragiaFibrinaHemostáticosHidrogelesHígadoInflamaciónMasculinoNanoestructurasNecrosisRatas WistarSefarosa10.1002/term.28311932-7005PMC6594136https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/term.2831https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594136/pdf