Martínez-Huélamo, MiriamTulipani, SaraJáuregui, OlgaValderas-Martínez, PalmiraVallverdú-Queralt, AnnaEstruch, RamónTorrado, XavierLamuela-Raventós, Rosa M2016-08-102016-08-102015-11-16Martínez-Huélamo M, Tulipani S, Jáuregui O, Valderas-Martínez P, Vallverdú-Queralt A, Estruch R, et al. Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples. Molecules. 2015; 20(11):20409-25http://hdl.handle.net/10668/2361Journal Article; Research Support, Non-U.S. Gov't;An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame.enPolyphenol metabolitesValidationTomatoSauceMicrobiotaMetabolitesBiosamplesBloodUrineFluidos corporalesCromatografía líquida de alta presiónVoluntarios sanosLímite de detecciónFenolesHumanosEspectrometría de masas en tándemMedical Subject Headings::Anatomy::Fluids and Secretions::Body FluidsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Chromatography::Chromatography, Liquid::Chromatography, High Pressure LiquidMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Sensitivity and Specificity::Limit of DetectionMedical Subject Headings::Technology, Industry, Agriculture::Food and Beverages::Food::Vegetables::Lycopersicon esculentumMedical Subject Headings::Chemicals and Drugs::Organic Chemicals::PhenolsMedical Subject Headings::Anatomy::Fluids and Secretions::Body Fluids::Blood::PlasmaMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Mass Spectrometry::Tandem Mass Spectrometryresearch article26580589open access10.3390/molecules2011197021420-3049