Serena, CarolinaQueipo-Ortuño, MaribelMillan, MonicaSanchez-Alcoholado, LidiaCaro, AleidisEspina, BeatrizMenacho, MargaritaBautista, MichelleMonfort-Ferré, DiandraTerrón-Puig, MargaridaNúñez-Roa, CatalinaMaymó-Masip, ElsaRodriguez, M. MarTinahones, Francisco J.Espin, EloyMartí, MarcFernández-Veledo, SoniaVendrell, Joan2022-08-022022-08-022020-07-31Serena C, Queipo-Ortuño M, Millan M, Sanchez-Alcoholado L, Caro A, Espina B, et al. Microbial Signature in Adipose Tissue of Crohn's Disease Patients. J Clin Med. 2020 Jul 31;9(8):2448http://hdl.handle.net/10668/3865Crohn's disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Tissue microbiotaInflammatory bowel disease16S sequencingCreeping fatPICRUSt analysisEscherichia coliFusobacteriumLipopolysaccharide biosynthesisMicrobiotaEnfermedad inflamatoria intestinalARN ribosómico 16SLipopolisacáridosTejidosEnfermedad de CrohnTejido adiposoMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::DysbiosisMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Albumins::C-Reactive ProteinMedical Subject Headings::Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, BacterialMedical Subject Headings::Chemicals and Drugs::Lipids::LipopolysaccharidesMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::HyperplasiaMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Nerve Tissue Proteins::S100 Proteins::Leukocyte L1 Antigen ComplexMedical Subject Headings::Organisms::Bacteria::ProteobacteriaMedical Subject Headings::Diseases::Pathological Conditions, Signs and Symptoms::Pathologic Processes::InflammationMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose TissueMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Health Status Indicators::Patient Acuity::Severity of Illness IndexMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Metabolism::Metabolic Networks and Pathwaysresearch article32751800open access10.3390/jcm90824482077-0383PMC7465250