Vidal, AngelaRios, RafaelPineda, CarmenLopez, IgnacioMuñoz-Castañeda, Juan R.Rodriguez, MarianoAguilera-Tejero, EscolasticoRaya, Ana I.2022-04-212022-04-212020-02-04Vidal A, Rios R, Pineda C, Lopez I, Muñoz-Castañeda JR, Rodriguez M, et al. Direct regulation of fibroblast growth factor 23 by energy intake through mTOR. Sci Rep. 2020 Feb 4;10(1):1795http://hdl.handle.net/10668/3556To test the hypothesis that fibroblast growth factor 23 (FGF23) is directly regulated by energy intake, in vivo and in vitro experiments were conducted. Three groups of rats were fed diets with high (HC), normal (NC) and low (LC) caloric content that resulted in different energy intake. In vitro, UMR106 cells were incubated in high (HG, 4.5 g/l) or low glucose (LG, 1 g/l) medium. Additional treatments included phosphorus (P), mannitol, rapamycin and everolimus. Intestinal absorption of P and plasma P concentrations were similar in the three groups of rats. As compared with NC, plasma FGF23 concentrations were increased in HC and decreased in the LC group. A significant correlation between energy intake and plasma FGF23 concentrations was observed. In vitro, mRNA FGF23 was significantly higher in UMR106 cells cultured in HG than in LG. When exposed to high P, mRNA FGF23 increased but only when cells were cultured in HG. Cells incubated with HG and mechanistic target of rapamycin (mTOR) inhibitors expressed low mRNA FGF23, similar to the values obtained in LG. In conclusion, this study shows a direct regulation of FGF23 production by energy availability and demonstrates that the mTOR signaling pathway plays a central role in this regulatory system.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/CellsRatsEnergy intakeRNA, messengerFibroblast growth factor-23GlucoseTOR serine-threonine kinasesCélulasRatasIngestión de energíaARN mensajeroFactores de crecimiento de fibroblastos 23GlucosaSerina-treonina quinasas TORMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Anatomy::Cells::Cells, Cultured::Cell Line::Cell Line, TumorMedical Subject Headings::Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Energy IntakeMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Fibroblast Growth FactorsMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Monosaccharides::Hexoses::GlucoseMedical Subject Headings::Chemicals and Drugs::Inorganic Chemicals::Elements::PhosphorusMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::RatsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats::Rats, WistarMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::TOR Serine-Threonine Kinasesresearch article32020002Acceso abierto10.1038/s41598-020-58663-72045-2322PMC7000745