Alcantara, J M AGalgani, J EJurado-Fasoli, LDote-Montero, MMerchan-Ramirez, ERavussin, ERuiz, J RSanchez-Delgado, G2023-05-032023-05-032022-05-18Alcantara JMA, Galgani JE, Jurado-Fasoli L, Dote-Montero M, Merchan-Ramirez E, Ravussin E, et al. Validity of four commercially available metabolic carts for assessing resting metabolic rate and respiratory exchange ratio in non-ventilated humans. Clin Nutr. 2022 Mar;41(3):746-754http://hdl.handle.net/10668/22112The validity of most commercially available metabolic cart is mostly unknown. Thus, we aimed to determine the accuracy, precision, within-subject reproducibility, and concordance of RMR and RER measured by four commercially available metabolic carts [Cosmed Q-NRG, Vyaire Vyntus CPX, Maastricht Instruments Omnical, and Medgraphics Ultima CardiO2]. Further, we studied whether a previously proposed simulation-based post-calorimetric calibration of cart readouts [individual calibration control evaluation (ICcE)] modify the RMR and RER reproducibility and concordance. Three experiments simulating different RMR and RER by controlled pure gas (N2 and CO2) infusions were conducted on 5 non-consecutive days. Moreover, 30-min methanol burns were performed on 3 non-consecutive days. Lastly, the RMR and RER of 29 young non-ventilated adults (11 women; 25 ± 4 years-old; BMI: 24.1 ± 3.2 kg/m2) were assessed twice using each instrument, 24 hours apart, under standardized conditions. The Omnical presented the lowest measurement error for RER (Omnical = 1.7 ± 0.9%; Vyntus = 4.5 ± 2.0%; Q-NRG = 6.6 ± 1.9%; Ultima = 6.8 ± 6.5%) and EE (Omnical = 1.5 ± 0.5%; Q-NRG = 2.5 ± 1.3%; Ultima = 10.7 ± 11.0%; Vyntus = 13.8 ± 5.0%) in all in vitro experiments (controlled pure gas infusions and methanol burns). In humans, the 4 metabolic carts provided discordant RMR and RER estimations (all P  The 4 metabolic carts provided discordant measurements of RMR and RER. Overall, the Omnical provides more accurate and precise estimations of RMR and RER than the Q-NRG, Vyntus and Ultima CardiO2, and might be considered the best for assessing RMR and RER in non-ventilated humans. Finally, our results do not support the use of an ICcE procedure.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Basal metabolic rateIndirect calorimetryReliabilityAdultBasal MetabolismCalorimetry, IndirectEnergy MetabolismFemaleHumansMaleMethanolReproducibility of ResultsYoung Adultresearch article35180452open accessAdultoCalorimetría indirectaFemeninoMasculinoMetabolismo basalMetabolismo energéticoMetanolReproducibilidad de los resultados10.1016/j.clnu.2022.01.0311532-1983PMC9616127https://digibug.ugr.es/bitstream/10481/74059/1/1-s2.0-S0261561422000383-main.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616127/pdf