Fernandez-Ruiz, MGimenez, EVinuesa, VRuiz-Merlo, TParra, PAmat, PMontejo, MPaez-Vega, ACantisan, STorre-Cisneros, JFortun, JAndres, ASan Juan, RLopez-Medrano, FNavarro, DAguado, J M2023-01-252023-01-252018-05-17Fernández-Ruiz M, Giménez E, Vinuesa V, Ruiz-Merlo T, Parra P, Amat P, et al. Regular monitoring of cytomegalovirus-specific cell-mediated immunity in intermediate-risk kidney transplant recipients: predictive value of the immediate post-transplant assessment. Clin Microbiol Infect. 2019 Mar;25(3):381.e1-381.e10http://hdl.handle.net/10668/12511Previous studies on monitoring of post-transplant cytomegalovirus (CMV)-specific cell-mediated immunity (CMI) are limited by single-centre designs and disparate risk categories. We aimed to assess the clinical value of a regular monitoring strategy in a large multicentre cohort of intermediate-risk kidney transplant (KT) recipients. We recruited 124 CMV-seropositive KT recipients with no T-cell-depleting induction pre-emptively managed at four Spanish institutions. CMV-specific interferon-γ-producing CD4+ and CD8+ T cells were counted through the first post-transplant year by intracellular cytokine staining after stimulation with pp65 and immediate early-1 peptides (mean of six measurements per patient). The primary outcome was the occurrence of any CMV event (asymptomatic infection and/or disease). Optimal cut-off values for CMV-specific T cells were calculated at baseline and day 15. Twelve-month cumulative incidence of CMV infection and/or disease was 47.6%. Patients with pre-transplant CMV-specific CD8+ T-cell count Monitoring for CMV-specific CMI in intermediate-risk KT recipients must be regular to reflect dynamic changes in overall immunosuppression and individual susceptibility. The early assessment at post-transplant day 15 remains particularly informative.enCell-mediated immunityCytomegalovirusImmune monitoring intracellular cytokine stainingKidney transplantationAgedCytomegalovirusCytomegalovirus infectionsFemaleHumansImmunity, cellularInterferon-gammaKidney transplantationLymphocyte countMaleMiddle agedMonitoring, immunologicPredictive value of testsRisk factorsT-lymphocytesTransplant recipientsResearch article29803844open accessCitomegalovirusFactores de riesgoInfecciones por citomegalovirusInmunidad celularInterferón gammaLinfocitos TMonitorización inmunológicaTrasplante de riñón10.1016/j.cmi.2018.05.0101469-0691http://www.clinicalmicrobiologyandinfection.com/article/S1198743X18304415/pdf