Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals.

Vargas, S LPonce, CBustamante, RCalderón, ENevez, GDe Armas, YMatos, OMiller, R FGallo, M J2023-01-252023-01-252017-06-05http://hdl.handle.net/10668/11275To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/AutopsyHumansLungMicrobiological TechniquesPneumocystisPneumonia, PneumocystisSpecimen HandlingImportance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals.research article28584896open access10.1007/s10096-017-3006-81435-4373PMC5602097https://link.springer.com/content/pdf/10.1007%2Fs10096-017-3006-8.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602097/pdf