Vargas, S LPonce, CBustamante, RCalderon, ENevez, GDe Armas, YMatos, OMiller, R FGallo, M J2023-01-252023-01-252017-10Vargas SL, Ponce C, Bustamante R, Calderón E, Nevez G, De Armas Y, et al. Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals. Eur J Clin Microbiol Infect Dis. 2017 Oct;36(10):1711-1716.http://hdl.handle.net/10668/11275To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Autopsy LungLung Tissue WeightPulmonary ColonizationJiroveciiChilled SerumAutopsyHumansLungMicrobiological TechniquesPneumocystisPneumonia, PneumocystisSpecimen Handlingreview28584896open accessMétodosDiagnósticoPneumocystisPulmónAutopsiaADN10.1007/s10096-017-3006-81435-4373PMC5602097https://link.springer.com/content/pdf/10.1007%2Fs10096-017-3006-8.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602097/pdf