Kofanova, OlgaBellora, CamilleQuesada, Rocio AguilarBulla, AlexandrePanadero-Fajardo, SoniaKeipes, MarcShea, KathiStone, MarsLescuyer, PierreBetsou, Fay2023-01-252023-01-252018-11-26http://hdl.handle.net/10668/13258Uncontrolled preanalytical variables can reduce the accuracy and reproducibility of downstream analytical results from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from EDTA and citrate-anticoagulated blood samples, obtained from healthy subjects and patients with inflammatory and infectious conditions. PBMC-derived RNA samples were examined for gene expression changes induced by extended blood pre-centrifugation delays at 4 °C and RT. We used Taqman RTqPCR to evaluate the combination of two target genes for their "diagnostic performance" in identifying EDTA and citrate-anticoagulated PBMC samples with extended pre-centrifugation times. We established the PBMC preanalytical score, a gene expression metric to asses the PBMC quality related to the pre-centrifugation delay at room temperature for different anticoagulants. The PBMC preanalytical score measurement can identify: CONCLUSION: The proposed PBMC preanalytical score may enable objective PBMC sample qualification for downstream applications, which may be influenced by blood precentrifugation delays.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/PBMCPreanalyticalQuality controlAdultAgedCalcium-Binding ProteinsFemaleGene Expression RegulationHumansInterleukin-8Leukocytes, MononuclearMaleMiddle Agedalpha-Mannosidaseresearch article30496732open access10.1016/j.jim.2018.11.0121872-7905https://doi.org/10.1016/j.jim.2018.11.012