Raue, RebeccaFrank, Ann-ChristinFuhrmann, Dominik Cde la Cruz-Ojeda, PatriciaRösser, SilviaBauer, RebekkaCardamone, GiuliaWeigert, AndreasSyed, Shahzad NawazSchmid, TobiasBrüne, Bernhard2023-05-032023-05-032022-02-222079-7737http://hdl.handle.net/10668/20800Macrophages constitute a major part of the tumor-infiltrating immune cells. Within the tumor microenvironment, they acquire an alternatively activated, tumor-supporting phenotype. Factors released by tumor cells are crucial for the recruitment of tumor-associated macrophages. In the present project, we aimed to understand the role of hsa-miR-200c-3p (miR-200c) in the interplay between tumor cells and macrophages. To this end, we employed a coculture system of MCF7 breast tumor cells and primary human macrophages and observed the transfer of miR-200c from apoptotic tumor cells to macrophages, which required intact CD36 receptor in macrophages. We further comprehensively determined miR-200c targets in macrophages by mRNA-sequencing and identified numerous migration-associated mRNAs to be downregulated by miR-200c. Consequently, miR-200c attenuated macrophage infiltration into 3-dimensional tumor spheroids. miR-200c-mediated reduction in infiltration further correlated with a miR-200c migration signature comprised of the four miR-200c-repressed, predicted targets PPM1F, RAB11FIB2, RDX, and MSN.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/breast tumormacrophagemiRtumor microenvironmentresearch article35336722open access10.3390/biology11030349PMC8945044https://www.mdpi.com/2079-7737/11/3/349/pdf?version=1645603260https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945044/pdf