Duell, Eric JLujan-Barroso, LeilaSala, NúriaDeitz McElyea, SamanthaOvervad, KimTjonneland, AnneOlsen, AnjaWeiderpass, ElisabeteBusund, Lill-ToveMoi, LineMuller, DavidVineis, PaoloAune, DagfinnMatullo, GiuseppeNaccarati, AlessioPanico, SalvatoreTagliabue, GiovannaTumino, RosarioPalli, DomenicoKaaks, RudolfKatzke, Verena ABoeing, HeinerBueno-de-Mesquita, H B AsPeeters, Petra HTrichopoulou, AntoniaLagiou, PagonaKotanidou, AnastasiaTravis, Ruth CWareham, NickKhaw, Kay-TeeRamon Quiros, JoseRodríguez-Barranco, MiguelDorronsoro, MirenChirlaque, María-DoloresArdanaz, EvaSeveri, GianlucaBoutron-Ruault, Marie-ChristineRebours, VincianeBrennan, PaulGunter, MarcScelo, GhislaineCote, GregSherman, StuartKorc, Murray2023-01-252023-01-252017-06-12http://hdl.handle.net/10668/11237Noninvasive biomarkers for early pancreatic ductal adenocarcinoma (PDAC) diagnosis and disease risk stratification are greatly needed. We conducted a nested case-control study within the Prospective Investigation into Cancer and Nutrition (EPIC) cohort to evaluate prediagnostic microRNAs (miRs) as biomarkers of subsequent PDAC risk. A panel of eight miRs (miR-10a, -10b, -21-3p, -21-5p, -30c, -106b, -155 and -212) based on previous evidence from our group was evaluated in 225 microscopically confirmed PDAC cases and 225 controls matched on center, sex, fasting status and age/date/time of blood collection. MiR levels in prediagnostic plasma samples were determined by quantitative RT-PCR. Logistic regression was used to model levels and PDAC risk, adjusting for covariates and to estimate area under the receiver operating characteristic curves (AUC). Plasma miR-10b, -21-5p, -30c and -106b levels were significantly higher in cases diagnosed within 2 years of blood collection compared to matched controls (all p-valuesenbiomarkerscohort studiesmicroRNAspancreatic cancerAdultAgedArea Under CurveBiomarkers, TumorCarcinoma, Pancreatic DuctalCase-Control StudiesFemaleHumansMaleMicroRNAsMiddle AgedPancreatic NeoplasmsPolymerase Chain ReactionProspective StudiesROC CurveSensitivity and Specificityresearch article28542740open access10.1002/ijc.307901097-0215PMC5536971https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ijc.30790https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536971/pdf