Ortiz-Alvarez, LAcosta, F MXu, HSanchez-Delgado, GVilchez-Vargas, RLink, APlaza-Diaz, JLlamas, J MGil, ALabayen, IRensen, P C NRuiz, J RMartinez-Tellez, B2023-05-032023-05-032022-10-07Ortiz-Alvarez L, Acosta FM, Xu H, Sanchez-Delgado G, Vilchez-Vargas R, Link A, et al. Fecal microbiota composition is related to brown adipose tissue 18F-fluorodeoxyglucose uptake in young adults. J Endocrinol Invest. 2023 Mar;46(3):567-576.http://hdl.handle.net/10668/21819Human brown adipose tissue (BAT) has gained considerable attention as a potential therapeutic target for obesity and its related cardiometabolic diseases; however, whether the gut microbiota might be an efficient stimulus to activate BAT metabolism remains to be ascertained. We aimed to investigate the association of fecal microbiota composition with BAT volume and activity and mean radiodensity in young adults. 82 young adults (58 women, 21.8 ± 2.2 years old) participated in this cross-sectional study. DNA was extracted from fecal samples and 16S rRNA sequencing was performed to analyse the fecal microbiota composition. BAT was determined via a static 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography-computed tomography scan (PET/CT) after a 2 h personalized cooling protocol. 18F-FDG uptake was also quantified in white adipose tissue (WAT) and skeletal muscles. The relative abundance of Akkermansia, Lachnospiraceae sp. and Ruminococcus genera was negatively correlated with BAT volume, BAT SUVmean and BAT SUVpeak (all rho ≤ - 0.232, P ≤ 0.027), whereas the relative abundance of Bifidobacterium genus was positively correlated with BAT SUVmean and BAT SUVpeak (all rho ≥ 0.262, P ≤ 0.012). On the other hand, the relative abundance of Sutterellaceae and Bifidobacteriaceae families was positively correlated with 18F-FDG uptake by WAT and skeletal muscles (all rho ≥ 0.213, P ≤ 0.042). All the analyses were adjusted for the PET/CT scan date as a proxy of seasonality. Our results suggest that fecal microbiota composition is involved in the regulation of BAT and glucose uptake by other tissues in young adults. Further studies are needed to confirm these findings. ClinicalTrials.gov no. NCT02365129 (registered 18 February 2015).enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Brown fatGlucose uptakeGut microbiotaObesityShort-chain fatty acidsAdultFemaleHumansYoung AdultAdipose Tissue, BrownCross-Sectional StudiesFluorodeoxyglucose F18MicrobiotaPositron Emission Tomography Computed TomographyPositron-Emission TomographyRNA, Ribosomal, 16Sresearch article36242744open accessARN Ribosómico 16SFluorodesoxiglucosa F18MicrobiotaTejido Adiposo PardoTomografía computarizada por tomografía de emisión de positronesHumanosFemeninoAdulto joven10.1007/s40618-022-01936-x1720-8386PMC9938059https://link.springer.com/content/pdf/10.1007/s40618-022-01936-x.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938059/pdf