Verwilt, JasperTrypsteen, WimVan-Paemel, RubenDe-Preter, KatleenGiraldez, Maria DMestdagh, PieterVandesompele, Jo2023-02-092023-02-092020-08-11Verwilt J, Trypsteen W, Van Paemel R, De Preter K, Giraldez MD, Mestdagh P, et al. When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies. Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):18934-18936.http://hdl.handle.net/10668/16096With great interest, we read the paper by Zhou et al. (1) describing a methodology that enables extracellular RNA sequencing (exRNA-seq) from extremely low input (Small Input Liquid Volume Extracellular RNA Sequencing [SILVER-seq]). We were intrigued by the high number of detected genes compared to our previous studies (2, 3) and noticed low reproducibility. We hypothesized that these observations could originate from substantial DNA contamination. Therefore, we reanalyzed the SILVER-seq data (4) to determine the extent of DNA signal in the sequencing reads.enExtracellular RNA analysisSequencing artifactsContaminant detectionData reproducibilityLiquid biopsy methodsLow-input sequencingDNADNA ContaminationHumansRNARNA-SeqExome Sequencingletter32788394Restricted AccessARN extracelularSecuenciación de ARNReproducibilidad de resultadosContaminación por ADNBioinformáticaSecuenciación de nucleótidos de alto rendimiento10.1073/pnas.20016751171091-6490PMC7431080https://www.pnas.org/content/pnas/117/32/18934.full.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431080/pdf