Cuevas, BelenArroba, Ana I.de los Reyes, CarolinaGomez-Jaramillo, LauraGonzalez-Montelongo, M. CarmenZubia, Eva2025-01-072025-01-072021-12-01https://hdl.handle.net/10668/25243Brown algae of the Family Dictyotaceae produce an array of structurally diverse terpenoids, whose biomedical potential in the anti-inflammatory area has been scarcely explored. Herein, the chemical study of the alga Rugulopteryx okamurae has led to the isolation of ten new diterpenoids: rugukadiol A (1), rugukamurals A-C (2-4), and ruguloptones A-F (6-10). The structures of the new compounds were established by spectroscopic means. Compound 1 exhibits an unprecedented diterpenoid skeleton featuring a bridged tricyclic undecane system. Compounds 2-10 belong to the secospatane class of diterpenoids and differ by the oxygenated functions that they contain. In anti-inflammatory assays, the new diterpenoid 1 and the secospatanes 5 and 10 significantly inhibited the production of the inflammatory mediator NO in LPS-stimulated microglial cells Bv.2 and macrophage cells RAW 264.7. Moreover, compounds 1 and 5 were found to strongly inhibit the expression of Nos2 and the pro-inflammatory cytokine Il1b in both immune cell lines.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/diterpenoidsbrown algaeRugulopteryx okamuraeanti-inflammatorynitric oxidecytokinemicrogliamacrophagesinvasive algaeFeeding-deterrent activityDictyota-menstrualisChinese collectionNitric-oxideMarine algaSeaweedSecospataneCancerresearch article34940676open access10.3390/md191206771660-3397https://www.mdpi.com/1660-3397/19/12/677/pdf?version=1638927573737718000001