Suarez, JuanKhom, SophiaAlen, FranciscoNatividad, Luis AVarodayan, Florence PPatel, Reesha RKirson, DeanArco, RocioBallesta, AntonioBajo, MichalRubio, LeticiaMartin-Fardon, RemiRodriguez de Fonseca, FernandoRoberto, Marisa2023-01-252023-01-252019-06-27Suárez J, Khom S, Alén F, Natividad LA, Varodayan FP, Patel RR, et al. Cessation of fluoxetine treatment increases alcohol seeking during relapse and dysregulates endocannabinoid and glutamatergic signaling in the central amygdala. Addict Biol. 2020 Sep;25(5):e12813http://hdl.handle.net/10668/14298Administration of selective serotonin reuptake inhibitors (SSRIs), typically used as antidepressants, induces long-lasting behavioral changes associated with alcohol use disorder (AUD). However, the contribution of SSRI (fluoxetine)-induced alterations in neurobiological processes underlying alcohol relapse such as endocannabinoid and glutamate signaling in the central amygdala (CeA) remains largely unknown. We utilized an integrative approach to study the effects of repeated fluoxetine administration during abstinence on ethanol drinking. Gene expression and biochemical and electrophysiological studies explored the hypothesis that dysregulation in glutamatergic and endocannabinoid mechanisms in the CeA underlie the susceptibility to alcohol relapse. Cessation of daily treatment with fluoxetine (10 mg/kg) during abstinence resulted in a marked increase in ethanol seeking during re-exposure periods. The increase in ethanol self-administration was associated with (a) reductions in levels of the endocannabinoids N-arachidonoylethanolomine and 2-arachidonoylglycerol in the CeA, (b) increased amygdalar gene expression of cannabinoid type-1 receptor (CB1), N-acyl phosphatidylethanolamine phospholipase D (Nape-pld), fatty acid amid hydrolase (Faah), (c) decreased amygdalar gene expression of ionotropic AMPA (GluA2 and GluA4) and metabotropic (mGlu3) glutamate receptors, and (d) increased glutamatergic receptor function. Overall, our data suggest that the administration of the antidepressant fluoxetine during abstinence dysregulates endocannabinoid signaling and glutamatergic receptor function in the amygdala, facts that likely facilitate alcohol drinking behavior during relapse.enAlcoholAmygdalaAntidepressantCannabinoidGlutamateRelapseAlcoholismAnimalsCentral amygdaloid nucleusDisease models, animalDrug-seeking behaviorEndocannabinoidsFluoxetineGlutamic acidMaleRatsRats, wistarRecurrenceSelective serotonin reuptake inhibitorsSignal transductionSubstance withdrawal syndromeresearch article31339221Restricted AccessAlcoholismoComportamiento de búsqueda de drogasEndocannabinoidesFluoxetinaNúcleo amigdalino centralSíndrome de abstinencia a sustanciasÁcido glutámico10.1111/adb.128131369-1600PMC8050940https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050940https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050940/pdf