Caro, CarlosGámez, FranciscoQuaresma, PedroPáez-Muñoz, Jose MaríaDomínguez, AlejandroPearson, John R.Pernía Leal, ManuelBeltrán, Ana M.Fernandez-Afonso, YilianDe la Fuente, Jesús M.Franco, RicardoPereira, EuláliaGarcía-Martín, Maria Luisa2022-12-132022-12-132021-03-20Caro C, Gámez F, Quaresma P, Páez-Muñoz JM, Domínguez A, Pearson JR, et al. Fe3O4-Au Core-Shell Nanoparticles as a Multimodal Platform for In Vivo Imaging and Focused Photothermal Therapy. Pharmaceutics. 2021 Mar 20;13(3):416http://hdl.handle.net/10668/4488In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable "light-to-heat" conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Imaging-guided therapyMultimodal imagingContrast agentMRICTPhotothermal therapyZebrafishTemperatureTissuesNanoparticlesImagen multimodalMedios de contrasteImagen por resonancia magnéticaTomografíacComputarizada por rayos XTerapia fototérmicaPez cebraTemperaturaTejidosNanopartículasNeoplasiasMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::MiceMedical Subject Headings::Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Diagnostic Uses of Chemicals::Contrast MediaMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Fishes::Cypriniformes::Cyprinidae::ZebrafishMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Surface Plasmon ResonanceMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Polymers::Plastics::Polyvinyls::PovidoneMedical Subject Headings::Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Atmosphere::Weather::Temperature::Hot TemperatureMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::RodentiaMedical Subject Headings::Phenomena and Processes::Metabolic Phenomena::Pharmacokinetics::Tissue DistributionMedical Subject Headings::Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles::Metal NanoparticlesMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Multimodal ImagingMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Tomography::Tomography, X-Ray::Tomography, X-Ray ComputedMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Magnetic Resonance ImagingMedical Subject Headings::Diseases::Neoplasmsresearch article33804636Acceso abierto10.3390/pharmaceutics130304161999-4923PMC8003746