Rodriguez-Perez, Maria DoloresPerez de Algaba, InmaculadaMartin-Aurioles, EstherArrebola, Maria MonsaludOrtega-Hombrados, LauraVerdugo, CristinaFernandez-Prior, Maria AfricaBermudez-Oria, AlejandraDe La Cruz, Jose PedroGonzalez-Correa, Jose Antonio2023-05-032023-05-032022-03-07Rodríguez-Pérez MD, Pérez de Algaba I, Martín-Aurioles E, Arrebola MM, Ortega-Hombrados L, Verdugo C, et al. Neuroprotective Effect of 3',4'-Dihydroxyphenylglycol in Type-1-like Diabetic Rats-Influence of the Hydroxytyrosol/3',4'-dihydroxyphenylglycol Ratio. Nutrients. 2022 Mar 8;14(6):1146http://hdl.handle.net/10668/21459The aim of this study was to assess the possible neuroprotective effect of 3',4'-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia-reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/3′,4′-dihydroxyphenylglycoldiabetes mellitusdiabetic neuropathyextra virgin olive oilhydroxytyrosolÁrea de Gestión Sanitaria Sur de CórdobaÁrea de Gestión Sanitaria Este de Málaga-AxarquíaAnimalsDiabetes Mellitus, experimentalNeuroprotective agentsPhenylethyl alcoholRatsRats, wistarresearch article35334801open accessAlcohol feniletílicoAnimalesDiabetes mellitus experimentalFármacos neuroprotectoresRatas wistar10.3390/nu140611462072-6643PMC8950825https://www.mdpi.com/2072-6643/14/6/1146/pdf?version=1646746841https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950825/pdf