Boughanem, HatimCabrera-Mulero, AmandaMillan-Gómez, MercedesGarrido-Sanchez, LourdesCardona, FernandoTinahones, Francisco JoseMoreno-Santos, InmaculadaMacias-Gonzalez, Manuel2023-01-252023-01-252020-11-15Boughanem H, Cabrera-Mulero A, Millán-Gómez M, Garrido-Sánchez L, Cardona F, Tinahones FJ, et al. Transcriptional Analysis of FOXO1, C/EBP-α and PPAR-γ2 Genes and Their Association with Obesity-Related Insulin Resistance. Genes (Basel). 2019 Sep 12;10(9):706http://hdl.handle.net/10668/14536Obesity is associated with several comorbid disorders, ranging from cardiovascular diseases to insulin resistance. In this context, visceral adipose tissue (VAT) seems to have a close connection with insulin resistance. In our study, we hypothesized that the expression profile of key adipogenic genes, such as proliferator-activated receptor γ type 2 (PPAR-γ2), CCAAT/enhancer-binding protein type α (C/EBP-α), and forkhead box protein class O type 1 (FOXO1) in VAT should shed light on their association with obesity-related insulin resistance. To test this idea, we studied the expression profile of C/EBP-α, FOXO1 and PPAR-γ2 in VAT from non-obese individuals, and low insulin (LIR-MO) and high insulin morbidly obese (HIR-MO) subjects, through a combination of RT-qPCR, co-immunoprecipitation, ELISA, Western blot analysis and EMSA assays. Our results show that C/EBP-α and PPAR-γ2 were down-expressed in HIR-MO individuals, while FOXO1 was overexpressed. In addition, the PPAR-γ2-RXR-α heterodimer showed weak activity and bound weakly to the putative IGFBP-2-PPRE promoter sequence in VAT from HIR-MO subjects when compared with LIR-MO individuals. These results show that PPAR-γ2, C/EBP-α, FOXO1 and IGFBP-2 have a close relationship with insulin resistance in VAT of morbidly obese individuals.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/C/EBP-αFOXO1IGFBP-2PPAR-γ2insulin resistanceobesityAdultAgedCCAAT-Enhancer-Binding Protein-alphaFemaleForkhead Box Protein O1HumansInsulin ResistanceInsulin-like growth factor binding protein 2MaleMiddle AgedObesity, MorbidPPAR gammaRNA, Messengerresearch article31547433open accessARN mensajeroObesidad mórbidaPPAR gammaPersona de mediana edadProteína forkhead box O1Proteína alfa potenciadora de unión a CCAATResistencia a la insulina10.3390/genes100907062073-4425PMC6770962https://www.mdpi.com/2073-4425/10/9/706/pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770962/pdf