Garzón, IngridJaimes-Parra, Boris DamiánPascual-Geler, ManriqueCózar, José ManuelSánchez-Quevedo, María del CarmenMosquera-Pacheco, María AuxiliadoraSánchez-Montesinos, IndalecioFernández-Valadés, RicardoCampos, FernandoAlaminos, Miguel2022-04-192022-04-192021-05-13Garzón I, Jaimes-Parra BD, Pascual-Geler M, Cózar J.M, Sánchez-Quevedo MC, Mosquera-Pacheco MA, et al. Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells. Polymers. 2021; 13(10):1568http://hdl.handle.net/10668/3538Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin-agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Urothelial mucosaHuman Wharton jelly mesenchymal stromal cellsBiofabricationMembrana mucosaEnsayo de materialesCélulas madre mesenquimatosasMedical Subject Headings::Chemicals and Drugs::Carbohydrates::Polysaccharides::SepharoseMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Cytoskeletal Proteins::Plakins::DesmoplakinsMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Uroplakins::Uroplakin IIMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Wharton JellyMedical Subject Headings::Anatomy::Urogenital System::Urinary Tract::UreterMedical Subject Headings::Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell DifferentiationMedical Subject Headings::Chemicals and Drugs::Biomedical and Dental Materials::Biocompatible MaterialsMedical Subject Headings::Anatomy::Tissues::Membranes::Mucous Membraneresearch article34068343open access10.3390/polym131015682073-4360PMC8153323