Jaafar, MariamContreras, JuliaDominique, CarineMartín-Villanueva, SaraCapeyrou, RegineVitali, PatriceRodriguez-Galan, OlgaVelasco, CarmenHumbert, OdileWatkins, Nicholas JVillalobo, EduardoBohnsack, Katherine EBohnsack, Markus THenry, YvesMerhi, Raghida Aboude-la-Cruz, JesusHenras, Anthony K2025-01-072025-01-072021-10-22Jaafar M, Contreras J, Dominique C, Martín-Villanueva S, Capeyrou R, Vitali P, et al. Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast. Nat Commun. 2021 Oct 22;12(1):6153.https://hdl.handle.net/10668/27566Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/RNARNA foldingSmall RNAsBase PairingDEAD-box RNA HelicasesMolecular ChaperonesMutationNuclear ProteinsOrganelle BiogenesisRNA FoldingRNA PrecursorsRNA, RibosomalRNA, Small NucleolarRibosome Subunits, Large, EukaryoticSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteinsresearch article34686656open accessPrecursores del ARNProteínasARN Nucleolar pequeñoRibosomasPeptidil TransferasasProteínas RibosómicasARN Ribosómico10.1038/s41467-021-26207-w2041-1723PMC8536666https://www.nature.com/articles/s41467-021-26207-w.pdfhttps://pmc.ncbi.nlm.nih.gov/articles/PMC8536666/pdf