Alvarez-Laderas, IsabelRamos, Teresa LMedrano, MayteCaracuel-García, RocíoBarbado, María VictoriaSánchez-Hidalgo, MarinaZamora, RosarioAlarcón-de-la-Lastra, CatalinaHidalgo, Francisco JPiruat, José IgnacioCaballero-Velázquez, TeresaPérez-Simón, José Antonio2023-02-082023-02-082019-11-19http://hdl.handle.net/10668/14733Polyphenols are a group of chemical substances found in plants, with immunomodulatory, antiproliferative, and anti-inflammatory properties that might be useful in the prophylaxis and treatment of graft-versus-host disease (GVHD). Polyphenolic extract (PE) obtained from extra virgin olive oil (EVOO) decreased the activation and proliferation of activated T cells. In addition, a decreased production of proinflammatory cytokines was observed upon exposure to PE. Western blot assays showed a marked inhibition of Akt phosphorylation and nuclear translocation of NF-κB in activated T cells. In a murine model of acute GVHD, we observed that mice that received a diet supplemented in PE (600 ppm) presented a higher survival rate and lower risk of developing GVHD when compared with the group that received a control diet. Histopathologic examination showed a significantly lower gut involvement in mice receiving PE, with a decrease in proinflammatory cytokines (IL-2, IL-17, and TNF-α) in serum and the reestablishment of butyrate concentration in the gut. In conclusion, PE obtained from EVOO exerted a potent immunomodulatory effect, reducing the activation and proliferation of activated T cells and the production of proinflammatory cytokines. In a murine model of acute GVHD, a PE-supplemented diet reduced the incidence and severity of the disease and increased survival after transplantation.enAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Graft-versus-host diseaseMouse modelPolyphenolic extractVirgin olive oilAnimalsDisease Models, AnimalGraft vs Host DiseaseMiceNF-kappa BOlive OilPlant Extractsresearch article31756538open access10.1016/j.bbmt.2019.11.0191523-6536https://doi.org/10.1016/j.bbmt.2019.11.019