Sanbonmatsu-Gámez, SaraPérez-Ruiz, MercedesCollao, XimenaSánchez-Seco, María PazMorillas-Márquez, FranciscoRosa-Fraile, Manuel de laNavarro-Marí, José MaríaTenorio, Antonio2012-11-082012-11-082005-11Sanbonmatsu-Gámez S, Pérez-Ruiz M, Collao X, Sánchez-Seco MP, Morillas-Márquez F, Rosa-Fraile M de la, et al. Toscana virus in Spain.Emerg Infect Dis. 2005 Nov;11(11):1701-7.1080-6040http://hdl.handle.net/10668/608Journal Article; Research Support, Non-U.S. Gov't;Toscana virus (TOSV, Phlebovirus, family Bunyaviridae) infection is one of the most prevalent arboviruses in Spain. Within the objectives of a multidisciplinary network, a study on the epidemiology of TOSV was conducted in Granada, in southern Spain. The overall seroprevalence rate was 24.9%, significantly increasing with age. TOSV was detected in 3 of 103 sandfly pools by viral culture or reverse transcription-polymerase chain reaction from a region of the L gene. Nucleotide sequence homology was 99%-100% in TOSV from vectors and patients and 80%-81% compared to the Italian strain ISS Phl.3. Sequencing of the N gene of TOSV isolates from patients and vectors indicated 87%-88% and 100% homology at the nucleotide and amino acid levels, respectively, compared to the Italian strain. These findings demonstrate the circulation of at least 2 different lineages of TOSV in the Mediterranean basin, the Italian lineage and the Spanish lineage.enAnimalesAnticuerpos AntiviralesFemeninoHumanosInmunoglobulina GInsectos VectoresMasculinoPhlebotomusFiebre por FlebótomosFilogeniaPrevalenciaVirus de Nápoles de la Fiebre de la Mosca de los ArenalesAnálisis de Secuencia de ADNEspañaProteínas ViralesMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, ViralMedical Subject Headings::Check Tags::FemaleMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin GMedical Subject Headings::Health Care::Environment and Public Health::Public Health::Disease Transmission, Infectious::Disease Vectors::Arthropod Vectors::Insect VectorsMedical Subject Headings::Check Tags::MaleMedical Subject Headings::Organisms::Eukaryota::Animals::Invertebrates::Arthropods::Insects::Diptera::Psychodidae::PhlebotomusMedical Subject Headings::Diseases::Virus Diseases::Arbovirus Infections::Phlebotomus FeverMedical Subject Headings::Phenomena and Processes::Genetic Phenomena::PhylogenyMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Vital Statistics::Morbidity::PrevalenceMedical Subject Headings::Organisms::Viruses::RNA Viruses::Bunyaviridae::Phlebovirus::Sandfly fever Naples virusMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNAMedical Subject Headings::Geographicals::Geographic Locations::Europe::SpainMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Viral Proteinsresearch article16318721open access10.3201/eid1111.0808511080-6059PMC3367369