Dahdah, NormaGonzalez-Franquesa, AlbaSamino, SaraGama-Perez, PauHerrero, LauraPerales, Jose CarlosYanes, OscarMalagon, Maria Del MarGarcia-Roves, Pablo Miguel2023-02-092023-02-092021-03-28Dahdah N, Gonzalez-Franquesa A, Samino S, Gama-Perez P, Herrero L, Perales JC, et al. Effects of Lifestyle Intervention in Tissue-Specific Lipidomic Profile of Formerly Obese Mice. Int J Mol Sci. 2021 Apr 1;22(7):3694http://hdl.handle.net/10668/17657Lipids are highly diverse in their composition, properties and distribution in different biological entities. We aim to establish the lipidomes of several insulin-sensitive tissues and to test their plasticity when divergent feeding regimens and lifestyles are imposed. Here, we report a proton nuclear magnetic resonance (1H-NMR) study of lipid abundance across 4 tissues of C57Bl6J male mice that includes the changes in the lipid profile after every lifestyle intervention. Every tissue analysed presented a specific lipid profile irrespective of interventions. Glycerolipids and fatty acids were most abundant in epididymal white adipose tissue (eWAT) followed by liver, whereas sterol lipids and phosphoglycerolipids were highly enriched in hypothalamus, and gastrocnemius had the lowest content in all lipid species compared to the other tissues. Both when subjected to a high-fat diet (HFD) and after a subsequent lifestyle intervention (INT), the lipidome of hypothalamus showed no changes. Gastrocnemius and liver revealed a pattern of increase in content in many lipid species after HFD followed by a regression to basal levels after INT, while eWAT lipidome was affected mainly by the fat composition of the administered diets and not their caloric density. Thus, the present study demonstrates a unique lipidome for each tissue modulated by caloric intake and dietary composition.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Diet compositionEnergy intakeExerciseGastrocnemiusHypothalamusLipidomicsLiverPlasticityTissue-specificWhite adipose tissueAdipose tissue, whiteAnimalsCaloric restrictionDiabetes mellitus, experimentalDiabetes mellitus, type 2Diet, high-fatDisease models, animalHealthy lifestyleHypothalamusLipidomicsLiverMaleMice, inbred C57BLMuscle, skeletalObesityPhysical conditioning, animalresearch article33916315open accessAnimalesCondicionamiento físico animalDiabetes mellitus experimentalDieta alta en grasaEstilo de vida saludableLipidómicaObesidad10.3390/ijms220736941422-0067PMC8037078https://www.mdpi.com/1422-0067/22/7/3694/pdf?version=1617939528https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037078/pdf