Claros, SilviaRodríguez-Losada, NoelaCruz, EncarnaciónGuerado, EnriqueBecerra, JoséAndrades, José A2013-01-142013-01-142012-04-02Claros, Silvia; Rodríguez-Losada, Noela; Cruz, Encarnación; Guerado, Enrique; Becerra, José; Andrades, José A. Characterization of adult stem/progenitor cell populations from bone marrow in a three-dimensional collagen gel culture system. Cell Transplantation. 2012;21(9):2021–20320963-6897http://hdl.handle.net/10668/735JOURNAL ARTICLE;Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells which are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and non-adherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4 and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent, hematopoietic, mesenchymal and endothelial specific markers in vitro and can undergo osteogenic differentiation in vivo.esDesmineralización Ósea PatológicaMolécula de Adhesión Celular del Leucocito ActivadoIntegrina alfa1Integrina alfa2Antígenos CD29Colágeno Tipo IAntígenos CD34Medical Subject Headings::Diseases::Musculoskeletal Diseases::Bone Diseases::Bone Diseases, Metabolic::Bone Demineralization, PathologicMedical Subject Headings::Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Esterases::Phosphoric Monoester Hydrolases::Nucleotidases::5'-NucleotidaseMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Cell Adhesion Molecules::Cell Adhesion Molecules, Neuronal::Cell Adhesion Molecules, Neuron-Glia::Activated-Leukocyte Cell Adhesion MoleculeMedical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Collagen::Integrin alpha1beta1Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Platelet Membrane Glycoproteins::Integrin alpha2beta1Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Integrins::Integrin beta Chains::Antigens, CD29Medical Subject Headings::Chemicals and Drugs::Macromolecular Substances::Polymers::Biopolymers::Collagen::Fibrillar Collagens::Collagen Type IMedical Subject Headings::Chemicals and Drugs::Biological Factors::Antigens::Antigens, Surface::Antigens, Differentiation::Antigens, CD::Antigens, CD34Medical Subject Headings::Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Glycoproteins::Membrane Glycoproteins::Cell Adhesion Molecules::Antigens, CD146research article22472743open access10.3727/096368912X6369391555-3892