Juhasz, BertalanPech-Puch, DawrinTabudravu, Jioji NCautain, BastienReyes, FernandoJiménez, CarlosKyeremeh, KwakuJaspars, Marcel2023-02-092023-02-092021-06-03http://hdl.handle.net/10668/18104Three dermacozines, dermacozines N-P (1-3), were isolated from the piezotolerant Actinomycete strain Dermacoccus abyssi MT 1.1T, which was isolated from a Mariana Trench sediment in 2006. Herein, we report the elucidation of their structures using a combination of 1D/2D NMR, LC-HRESI-MSn, UV-Visible, and IR spectroscopy. Further confirmation of the structures was achieved through the analysis of data from density functional theory (DFT)-UV-Visible spectral calculations and statistical analysis such as two tailed t-test, linear regression-, and multiple linear regression analysis applied to either solely experimental or to experimental and calculated 13C-NMR chemical shift data. Dermacozine N (1) bears a novel linear pentacyclic phenoxazine framework that has never been reported as a natural product. Dermacozine O (2) is a constitutional isomer of the known dermacozine F while dermacozine P (3) is 8-benzoyl-6-carbamoylphenazine-1-carboxylic acid. Dermacozine N (1) is unique among phenoxazines due to its near infrared (NIR) absorption maxima, which would make this compound an excellent candidate for research in biosensing chemistry, photodynamic therapy (PDT), opto-electronic applications, and metabolic mapping at the cellular level. Furthermore, dermacozine N (1) possesses weak cytotoxic activity against melanoma (A2058) and hepatocellular carcinoma cells (HepG2) with IC50 values of 51 and 38 μM, respectively.enAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/(DFT)-UV-Vis spectral calculation13C-NMR chemical shift linear and multiple regressionDermacoccus abyssi MT 1.1TMariana Trenchabsorption maxima in the near infrared regiondeep sea natural productsdermacozinephenoxazineActinobacteriaGeologic SedimentsLightMagnetic Resonance SpectroscopyPhenazinesPhotochemical ProcessesRegression AnalysisSpectrophotometryresearch article34205180open access10.3390/md190603251660-3397PMC8226881https://www.mdpi.com/1660-3397/19/6/325/pdf?version=1623238863https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226881/pdf