Montero-Vilchez, TrinidadSierra-Sánchez, ÁlvaroSanchez-Diaz, ManuelQuiñones-Vico, Maria IsabelSanabria-de-la-Torre, RaquelMartinez-Lopez, AntonioArias-Santiago, Salvador2022-09-272022-09-272021-07-23Montero-Vilchez T, Sierra-Sánchez Á, Sanchez-Diaz M, Quiñones-Vico MI, Sanabria-de-la-Torre R, Martinez-Lopez A, et al. Mesenchymal Stromal Cell-Conditioned Medium for Skin Diseases: A Systematic Review. Front Cell Dev Biol. 2021 Jul 23;9:654210http://hdl.handle.net/10668/4150The skin is the largest organ of the human body, and its dysfunction is related to many diseases. There is a need to find new potential effective therapies for some skin conditions such as inflammatory diseases, wound healing, or hair restoration. Mesenchymal stromal cell (MSC)-conditioned medium (CM) provides a potential opportunity in the treatment of skin disease. Thus, the objective of this review is to evaluate the uses of MSC-CM for treating skin diseases in both animal and human models. A systematic review was conducted regarding the use of MSC-CM for treating skin conditions. One hundred one studies were analyzed. MSC-CM was evaluated in wound healing (55), hypertrophic scars (9), flap reperfusion (4), hair restoration (15), skin rejuvenation (15), and inflammatory skin diseases (3). MSC-CM was obtained from different MSC sources, mainly adipose tissue, bone marrow, and umbilical cord blood. MSC-CM was tested intravenously, intraperitoneally, subcutaneously, intradermally or intralesionally injected or topically applied. MSC-CM was used in both animals and humans. MSC-CM improved wound healing, hair restoration, skin rejuvenation, atopic dermatitis, and psoriasis in both animals and humans. MSC-CM also decreased hypertrophic scars and flap ischemia in animal models. In conclusion, MSC-CM is a promising therapy for skin conditions. Further studies are needed to corroborate safety and effectiveness and to standardize CM manufacturing.enAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Advanced therapyConditioned mediumDermatologyMesenchymal stem cellsStem cellsMedios de cultivo condicionadosDermatologíaCélulas madre mesenquimatosasMedical Subject Headings::Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::HumansMedical Subject Headings::Organisms::Eukaryota::AnimalsMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Equipment and Supplies::Culture Media::Culture Media, ConditionedMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Granulation Tissue::Cicatrix::Cicatrix, HypertrophicMedical Subject Headings::Anatomy::Hemic and Immune Systems::Immune System::Bone MarrowMedical Subject Headings::Diseases::Skin and Connective Tissue Diseases::Skin Diseases::Dermatitis::Dermatitis, AtopicMedical Subject Headings::Anatomy::Fluids and Secretions::Body Fluids::Blood::Fetal BloodMedical Subject Headings::Anthropology, Education, Sociology and Social Phenomena::Social Sciences::Anthropology::Anthropology, Cultural::Culture::Human BodyMedical Subject Headings::Analytical, Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::RejuvenationMedical Subject Headings::Anatomy::Tissues::Connective Tissue::Adipose TissueMedical Subject Headings::Diseases::Skin and Connective Tissue Diseases::Skin Diseases::Skin Diseases, Papulosquamous::Psoriasisreview article34368115open access10.3389/fcell.2021.6542102296-634XPMC8343397